牛病毒性腹泻病毒感染对牛外周血来源树突状细胞表型及其分泌Th1/Th2细胞因子的影响

doi:10.11843/j.issn.0366-6964.2016.03.016

Abstract

Abstract:

The objective of this study was to investigate the influence of BVDV on the cell maturation and cytokine level of Th1 and Th2 type in dendritic cells (DC) derived from bovine peripheral blood，and to further reveal the immune mechanism of BVDV infection.The monocytes from bovine peripheral blood were separated and induced into DCs，and then were co-cultured with BVDV.The cell morphology and the expression of MHCⅡ，CD80 and CD40 on DCs were observed by microscopy and flow cytometry respectively，the semi-quantitative RT-PCR was used to analyze IL-12p40 of Th1 type cytokines and IL-10 of Th2 at mRNA levels.The results showed that DCs had typical morphology and immunophenotype with 93.81% purity.Post BVDV infection，the MHCII molecules，CD40 molecules and CD80 molecules were greatly lower than that of the control groups，the differences were statistically significant (P＜0.01). IL-12p40 mRNA level was down-regulated，while IL-10 mRNA level was up-regulated，the differences were significant (P＜0.05) compared with the control groups.These results indicated that BVDV infection to DC plays an important role on maturation and regulating the balance of Th1/Th2.

CLC Number:

S852.659.6

LIU Jin-ling，WEI Shu，ZHAO Yu-jun，HE Jian-bin. The Influence of BVDV on Immunophenotype and Th1/Th2 Cytokine Secretion of Bovine Peripheral Blood-derived Dendritic Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(3): 536-542.

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References

［1］韩猛立，李娜，黄新，等.牛病毒性腹泻病毒巢式RT-PCR检测方法的建立及应用［J］.西北农业学报，2010，19(10)：6-9,15.
HAN M L，LI N，HUANG X，et al.Establishment and application of nested PCR assay for bovine viral diarrhea virus detection［J］.Acta Agriculturae Boreali-Occidentalis Sinica， 2010，19(10)：6-9,15.(in Chinese)
［2］韩猛立，黄新，宋天增，等.牛病毒性腹泻病毒与宿主细胞的相互作用研究［J］.湖北农业科学，2012，51（8）：1519-1523.
HAN M L，HUANG X，SONG T Z，et al.Research progress of interactions between bovine viral diarrhea virus and host cell［J］.Hubei Agricultural Sciences，2012，51（8）：1519-1523.(in Chinese)
［3］GLEW E J，CARR B V，BRACKENBURY L S，et al.Differential effects of bovine viral diarrhoea virus on monocytes and dendritic cells［J］.J Gen Virol，2003,84 (Pt 7)：1771-1780.
［4］LEE S R，NANDURI B，PHARR G T，et al.Bovine viral diarrhea virus infection affects the expression of proteins related to professional antigen presentation in bovine monocytes［J］.Biochim Biophys Acta，2009，1794(1)：14-22.
［5］TOPLIFF C L，SMITH D R，CLOWSER S L，et al.Prevalence of bovine viral diarrhea virus infections in alpacas in the united states［J］.J Am Vet Med Assoc，2009，234(4)：519-529.
［6］BYERS S R，SNEKVIK K R，RIGHTER D J，et al.Disseminated Bovine viral diarrhea virus in a persistently infected alpaca(Vicugna pacos) cria［J］.J Vet Diagn Invest，2009，21(1)：145-148.
［7］朱诗语，王春凤，杨桂连.树突状细胞在病毒感染动物免疫应答中的作用［J］.畜牧与兽医，2014，46(12)：106-108.
ZHU S Y，WANG C F，YANG G L.The role of dendritic cells on animal immune response against virus infection［J］.Animal Husbandry and Veterinary Medicine，2014，46(12)：106-108.(in Chinese)
［8］PINCHUK G V，LEE S R，NANDURI B，et al.Bovine viral diarrhea viruses differentially alter the expression of the protein kinases and related proteins affecting the development of infection and anti-viral mechanisms in bovine monocytes［J］.Biochim Biophys Acts，2008，1784(9)：1234-1247.
［9］SALMOND R J，ZAMOYSKA R.The influence of mTOR on T helper cell differentiation and dendritic cell function［J］.Eur J Immunol，2011，41(8)：2137-2141.
［10］LEE K，GUDAPATI P，DRAGOVIC S,et al.Mammalian target of rapamycin protein complex 2 regulates differentiation of Th1 and Th2 cell subsets via distinct signaling pathways［J］.Immunity，2010，32(6)：743-753.
［11］MORRIS S，SWANSON M S，LIEBERMAN A,et al.Autophagy-mediated dendritic cell activation is essential for innate cytokine production and APC function with respiratory syncytial virus responses［J］.J Immunol，2011，187(8)：3953-3961.
［12］LEE S R，PHARR G T，BOYD B L，et al.Bovine viral diarrhea viruses modulate toll-like receptors，cytokines and co-stimulatory molecules gene expression in bovine peripheral blood monocytes［J］.Comp Immunol Microbiol Infect Dis，2008，31(5)：403-418.
［13］NEURATH M F，FINOTTO S，GLIMCHER L H.The role of Th1/Th2 polarization in mucosal immunity［J］.Nat Med，2002，8(6)：567-573.

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The Influence of BVDV on Immunophenotype and Th1/Th2 Cytokine Secretion of Bovine Peripheral Blood-derived Dendritic Cells

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